1、照氣相色譜法（中國(guó)藥典2000年版二部附錄VE）測(cè)定。
2、色譜條件與系統(tǒng)適用性試驗(yàn)：以己二酸乙醇酯為固定相，涂布濃度為0.5%，柱溫為60℃，檢測(cè)器為氫火焰離子化檢測(cè)器，大蒜素峰與內(nèi)標(biāo)物質(zhì)峰的分離度應(yīng)符合要求。校正因子測(cè)定取苯己酮適量。加正己烷溶解并稀釋?zhuān)瞥擅?批中含10mg的溶液，作為內(nèi)標(biāo)溶液。另取三硫二丙烯對(duì)照品約30mg，精密稱(chēng)定，置10ml容量瓶中，精密加入內(nèi)標(biāo)溶液1ml，加正己烷稀釋至刻度，搖勻，取1-2ml注入氣相色譜儀，計(jì)算校正因子。
3、測(cè)定法：精密吸取本品適量（約相當(dāng)于三硫二丙烯30mg），置分液漏斗中，用正己烷振搖萃取3次（每次2ml），分出正己烷層，置10ml容量瓶中，精密加入內(nèi)標(biāo)溶液1ml，加正己烷稀釋至刻度，搖勻，取1-2UL注入氣相色譜儀，測(cè)定，計(jì)算。
浩瀚色譜（山東）應(yīng)用技術(shù)開(kāi)發(fā)有限公司，研發(fā)氣相色譜法測(cè)定藥典大蒜素的含量，面積內(nèi)標(biāo)法定量，結(jié)果滿意。
名稱(chēng)：填充柱
型號(hào)：0．5％的己二酸乙二 醇酯/Chromosorb W AW
規(guī)格：2m*1/8，80-100目
*高使用溫度：200°C
應(yīng)用：藥典2000年版 大蒜素 氣相色譜法
1. Determine according to the gas chromatography method (Appendix VE of Part II of the Chinese Pharmacopoeia, 2000 edition).
2. Chromatographic conditions and system suitability test: Using ethanol adipate as the stationary phase, with a coating concentration of 0.5% and a column temperature of 60 ℃, the detector is a hydrogen flame ionization detector. The separation degree between the allicin peak and the internal standard substance peak should meet the requirements. Measure the correction factor by taking an appropriate amount of benzophenone. Dissolve and dilute with n-hexane to prepare a solution containing 10mg per batch as the internal standard solution. Take another 30mg of tripropene reference substance, weigh it precisely, put it into a 10ml volumetric flask, add 1ml of internal standard solution precisely, add n-hexane to dilute it to the scale, shake it up, take 1-2ml of it and inject it into the gas chromatograph to calculate the correction factor.
3. Determination method: Precisely suck an appropriate amount of this product (about 30mg of tripropene), place it in a separatory funnel, shake and extract it three times with n-hexane (2ml each time), separate the n-hexane layer, place it in a 10ml volumetric flask, precisely add 1ml of internal standard solution, add n-hexane to dilute it to the scale, shake it up, take 1-2UL and inject it into the gas chromatograph, measure and calculate.
Haohan Chromatography (Shandong) Application Technology Development Co., Ltd. has developed a gas chromatography method to determine the content of allicin in the pharmacopoeia, and an area internal standard method for quantification, with satisfactory results.
Name: Filled Column
Model: 0.5% ethylene glycol adipate/Chromosorb W AW
Specification: 2m * 1/8, 80-100 mesh
*High operating temperature: 200 ° C
Application: Pharmacopoeia 2000 Edition Garlic Gas Chromatography
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